The potential of metabolite profiling as a selection tool for genotype discrimination in Populus.
Identifieur interne : 003F37 ( Main/Exploration ); précédent : 003F36; suivant : 003F38The potential of metabolite profiling as a selection tool for genotype discrimination in Populus.
Auteurs : Andrew R. Robinson [Canada] ; Rana Gheneim ; Robert A. Kozak ; Dave D. Ellis ; Shawn D. MansfieldSource :
- Journal of experimental botany [ 0022-0957 ] ; 2005.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Analyse en composantes principales (MeSH), Chromatographie en phase liquide à haute performance (MeSH), Chromatographie gazeuse-spectrométrie de masse (MeSH), Génotype (MeSH), Lignine (analyse), Lignine (biosynthèse), Lignine (composition chimique), Phénotype (MeSH), Populus (classification), Populus (génétique), Populus (métabolisme), Régulation de l'expression des gènes végétaux (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- analyse : Lignine.
- biosynthèse : Lignine.
- composition chimique : Lignine, Populus.
- génétique : Populus, Régulation de l'expression des gènes végétaux.
- métabolisme : Populus.
- Analyse de profil d'expression de gènes, Analyse en composantes principales, Chromatographie en phase liquide à haute performance, Chromatographie gazeuse-spectrométrie de masse, Génotype, Phénotype, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid (MeSH), Gas Chromatography-Mass Spectrometry (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (genetics), Genotype (MeSH), Lignin (analysis), Lignin (biosynthesis), Lignin (chemistry), Phenotype (MeSH), Plants, Genetically Modified (MeSH), Populus (classification), Populus (genetics), Populus (metabolism), Principal Component Analysis (MeSH).
- MESH :
- chemical , analysis : Lignin.
- chemical , biosynthesis : Lignin.
- chemical , chemistry : Lignin.
- classification : Populus.
- genetics : Gene Expression Regulation, Plant, Populus.
- metabolism : Populus.
- Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry, Gene Expression Profiling, Genotype, Phenotype, Plants, Genetically Modified, Principal Component Analysis.
Abstract
Differences between wild-type Populus tremulaxalba and two transgenic lines with modified lignin monomer composition, were interrogated using metabolic profiling. Analysis of metabolite abundance data by GC-MS, coupled with principal components analysis (PCA), successfully differentiated between lines that had distinct phenotypes, whether samples were taken from the cambial zone or non-lignifying suspension tissue cultures. Interestingly, the GC-MS analysis detected relatively few phenolic metabolites in cambial extracts, although a single metabolite associated with the differentiation between lines was directly related to the phenylpropanoid pathway or other down-stream aspects of lignin biosynthesis. In fact, carbohydrates, which have only an indirect relationship with the modified lignin monomer composition, featured strongly in the line-differentiating aspects of the statistical analysis. Traditional HPLC analysis was employed to verify the GC-MS data. These findings demonstrate that metabolic traits can be dissected reliably and accurately by metabolomic analyses, enabling the discrimination of individual genotypes of the same tree species that exhibit marked differences in industrially relevant wood traits. Furthermore, this validates the potential of using metabolite profiling techniques for marker generation in the context of plant/tree breeding for industrial applications.
DOI: 10.1093/jxb/eri273
PubMed: 16143717
Affiliations:
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Le document en format XML
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Robinson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Gheneim, Rana" sort="Gheneim, Rana" uniqKey="Gheneim R" first="Rana" last="Gheneim">Rana Gheneim</name></author><author><name sortKey="Kozak, Robert A" sort="Kozak, Robert A" uniqKey="Kozak R" first="Robert A" last="Kozak">Robert A. Kozak</name></author><author><name sortKey="Ellis, Dave D" sort="Ellis, Dave D" uniqKey="Ellis D" first="Dave D" last="Ellis">Dave D. Ellis</name></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. 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Analysis of metabolite abundance data by GC-MS, coupled with principal components analysis (PCA), successfully differentiated between lines that had distinct phenotypes, whether samples were taken from the cambial zone or non-lignifying suspension tissue cultures. Interestingly, the GC-MS analysis detected relatively few phenolic metabolites in cambial extracts, although a single metabolite associated with the differentiation between lines was directly related to the phenylpropanoid pathway or other down-stream aspects of lignin biosynthesis. In fact, carbohydrates, which have only an indirect relationship with the modified lignin monomer composition, featured strongly in the line-differentiating aspects of the statistical analysis. Traditional HPLC analysis was employed to verify the GC-MS data. These findings demonstrate that metabolic traits can be dissected reliably and accurately by metabolomic analyses, enabling the discrimination of individual genotypes of the same tree species that exhibit marked differences in industrially relevant wood traits. Furthermore, this validates the potential of using metabolite profiling techniques for marker generation in the context of plant/tree breeding for industrial applications.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16143717</PMID><DateCompleted><Year>2006</Year><Month>01</Month><Day>10</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0022-0957</ISSN><JournalIssue CitedMedium="Print"><Volume>56</Volume><Issue>421</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>The potential of metabolite profiling as a selection tool for genotype discrimination in Populus.</ArticleTitle><Pagination><MedlinePgn>2807-19</MedlinePgn></Pagination><Abstract><AbstractText>Differences between wild-type Populus tremulaxalba and two transgenic lines with modified lignin monomer composition, were interrogated using metabolic profiling. Analysis of metabolite abundance data by GC-MS, coupled with principal components analysis (PCA), successfully differentiated between lines that had distinct phenotypes, whether samples were taken from the cambial zone or non-lignifying suspension tissue cultures. Interestingly, the GC-MS analysis detected relatively few phenolic metabolites in cambial extracts, although a single metabolite associated with the differentiation between lines was directly related to the phenylpropanoid pathway or other down-stream aspects of lignin biosynthesis. In fact, carbohydrates, which have only an indirect relationship with the modified lignin monomer composition, featured strongly in the line-differentiating aspects of the statistical analysis. Traditional HPLC analysis was employed to verify the GC-MS data. These findings demonstrate that metabolic traits can be dissected reliably and accurately by metabolomic analyses, enabling the discrimination of individual genotypes of the same tree species that exhibit marked differences in industrially relevant wood traits. Furthermore, this validates the potential of using metabolite profiling techniques for marker generation in the context of plant/tree breeding for industrial applications.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Robinson</LastName><ForeName>Andrew R</ForeName><Initials>AR</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gheneim</LastName><ForeName>Rana</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Kozak</LastName><ForeName>Robert A</ForeName><Initials>RA</Initials></Author><Author ValidYN="Y"><LastName>Ellis</LastName><ForeName>Dave D</ForeName><Initials>DD</Initials></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>09</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008401" MajorTopicYN="N">Gas Chromatography-Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="Y">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025341" MajorTopicYN="N">Principal Component Analysis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>9</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>1</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>9</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16143717</ArticleId><ArticleId IdType="pii">eri273</ArticleId><ArticleId IdType="doi">10.1093/jxb/eri273</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Ellis, Dave D" sort="Ellis, Dave D" uniqKey="Ellis D" first="Dave D" last="Ellis">Dave D. Ellis</name><name sortKey="Gheneim, Rana" sort="Gheneim, Rana" uniqKey="Gheneim R" first="Rana" last="Gheneim">Rana Gheneim</name><name sortKey="Kozak, Robert A" sort="Kozak, Robert A" uniqKey="Kozak R" first="Robert A" last="Kozak">Robert A. Kozak</name><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Robinson, Andrew R" sort="Robinson, Andrew R" uniqKey="Robinson A" first="Andrew R" last="Robinson">Andrew R. Robinson</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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